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pmscv neo retroviral vector backbone  (TaKaRa)


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    TaKaRa pmscv neo retroviral vector backbone
    Pmscv Neo Retroviral Vector Backbone, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 15972 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/backbone+retroviral+vector/EcoR+I/pmc03364258-206-9-13
    Average 99 stars, based on 15972 article reviews
    pmscv neo retroviral vector backbone - by Bioz Stars, 2026-09
    99/100 stars

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    Article Title: Multiplex T Cell Stimulation Assay Utilizing a T Cell Activation Reporter-Based Detection System
    Article Snippet: Nuclear factor of activated T cells-reporter genes were inserted in a retroviral vector that contains an inactivated 3′LTR. .. We generated a backbone retroviral vector in which the CMV 5′LTR and inactivated MoMuLV 3′LTR were replaced with the 5′LTR and 3′LTR regions of the murine stem cell virus (MSCV) vector (Clontech MSCV Retroviral Expression System). ..

    Retroviral:

    Article Title: Multiplex T Cell Stimulation Assay Utilizing a T Cell Activation Reporter-Based Detection System
    Article Snippet: Nuclear factor of activated T cells-reporter genes were inserted in a retroviral vector that contains an inactivated 3′LTR. .. We generated a backbone retroviral vector in which the CMV 5′LTR and inactivated MoMuLV 3′LTR were replaced with the 5′LTR and 3′LTR regions of the murine stem cell virus (MSCV) vector (Clontech MSCV Retroviral Expression System). ..

    Plasmid Preparation:

    Article Title: Multiplex T Cell Stimulation Assay Utilizing a T Cell Activation Reporter-Based Detection System
    Article Snippet: Nuclear factor of activated T cells-reporter genes were inserted in a retroviral vector that contains an inactivated 3′LTR. .. We generated a backbone retroviral vector in which the CMV 5′LTR and inactivated MoMuLV 3′LTR were replaced with the 5′LTR and 3′LTR regions of the murine stem cell virus (MSCV) vector (Clontech MSCV Retroviral Expression System). ..

    Virus:

    Article Title: Multiplex T Cell Stimulation Assay Utilizing a T Cell Activation Reporter-Based Detection System
    Article Snippet: Nuclear factor of activated T cells-reporter genes were inserted in a retroviral vector that contains an inactivated 3′LTR. .. We generated a backbone retroviral vector in which the CMV 5′LTR and inactivated MoMuLV 3′LTR were replaced with the 5′LTR and 3′LTR regions of the murine stem cell virus (MSCV) vector (Clontech MSCV Retroviral Expression System). ..



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    ANKH transcript is upregulated in PEsen human fibroblasts and downregulated by telomerase. (A) Expression of ANKH mRNA in growing and PEsen NHOF-1 cells using primer set 1. (B) This is similar to (A) but shows the results of primer set 2 in the same experiments. (C) This is similar to (A) but shows the expression of the senescence marker p16INK4A in the same experiments. (D) This is similar to (A) but shows the expression of the senescence marker p21WAF1 in the same experiments. (E) This is similar to (A) but shows SA-βGal expression. (A–E) Yellow plain bars, growing young cells 20.2-21 MPDs; blue horizontal striped bars, PEsen cells 68.2 MPDs. *, P < 0.05; **, P < 0.01; ***, P < 0.001; *P > 0.05 < 0.1; ns, not significant. The results are presented as the averages +/- standard deviation. N = 3. (F) Expression of ANKH mRNA in NHOF-1 PURO, <t>TERT-HA,</t> <t>DNTERT,</t> and TERT cell using primer set 1. N = 3. (G) This is similar to (F) but shows the results of primer set 2 in the same experiments. (H) This is similar to (F) but shows the expression of the senescence marker p16INK4A in the same experiments. (I) This is similar to (F) but shows the expression of the senescence marker p21WAF1 in the same experiments. (J) This is similar to (F) but shows SA-βGal expression. (K,L) Representative images of SA-βGal staining in (E). (K) Young growing NHOF-1 fibroblasts (20.2 MPDs). (L) . NHOF-1 cells (68.2 MPDs). Bar, 100 µm. (F–J) (A–E) Plain yellow bars, LATE PURO; blue horizontal striped bars, TERT-HA; brown stippled bars, DNTERT; and purple left-right diagonally striped bars, TERT all after completing 60 MPDs. *, P < 0.05; **, P < 0.01; ***, P < 0.001; * >0.05 < 0.1; ns, not significant. The results are presented as the averages +/- standard deviation. N = 4, except (J) .
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    ANKH transcript is upregulated in PEsen human fibroblasts and downregulated by telomerase. (A) Expression of ANKH mRNA in growing and PEsen NHOF-1 cells using primer set 1. (B) This is similar to (A) but shows the results of primer set 2 in the same experiments. (C) This is similar to (A) but shows the expression of the senescence marker p16INK4A in the same experiments. (D) This is similar to (A) but shows the expression of the senescence marker p21WAF1 in the same experiments. (E) This is similar to (A) but shows SA-βGal expression. (A–E) Yellow plain bars, growing young cells 20.2-21 MPDs; blue horizontal striped bars, PEsen cells 68.2 MPDs. *, P < 0.05; **, P < 0.01; ***, P < 0.001; *P > 0.05 < 0.1; ns, not significant. The results are presented as the averages +/- standard deviation. N = 3. (F) Expression of ANKH mRNA in NHOF-1 PURO, <t>TERT-HA,</t> <t>DNTERT,</t> and TERT cell using primer set 1. N = 3. (G) This is similar to (F) but shows the results of primer set 2 in the same experiments. (H) This is similar to (F) but shows the expression of the senescence marker p16INK4A in the same experiments. (I) This is similar to (F) but shows the expression of the senescence marker p21WAF1 in the same experiments. (J) This is similar to (F) but shows SA-βGal expression. (K,L) Representative images of SA-βGal staining in (E). (K) Young growing NHOF-1 fibroblasts (20.2 MPDs). (L) . NHOF-1 cells (68.2 MPDs). Bar, 100 µm. (F–J) (A–E) Plain yellow bars, LATE PURO; blue horizontal striped bars, TERT-HA; brown stippled bars, DNTERT; and purple left-right diagonally striped bars, TERT all after completing 60 MPDs. *, P < 0.05; **, P < 0.01; ***, P < 0.001; * >0.05 < 0.1; ns, not significant. The results are presented as the averages +/- standard deviation. N = 4, except (J) .
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    ANKH transcript is upregulated in PEsen human fibroblasts and downregulated by telomerase. (A) Expression of ANKH mRNA in growing and PEsen NHOF-1 cells using primer set 1. (B) This is similar to (A) but shows the results of primer set 2 in the same experiments. (C) This is similar to (A) but shows the expression of the senescence marker p16INK4A in the same experiments. (D) This is similar to (A) but shows the expression of the senescence marker p21WAF1 in the same experiments. (E) This is similar to (A) but shows SA-βGal expression. (A–E) Yellow plain bars, growing young cells 20.2-21 MPDs; blue horizontal striped bars, PEsen cells 68.2 MPDs. *, P < 0.05; **, P < 0.01; ***, P < 0.001; *P > 0.05 < 0.1; ns, not significant. The results are presented as the averages +/- standard deviation. N = 3. (F) Expression of ANKH mRNA in NHOF-1 PURO, <t>TERT-HA,</t> <t>DNTERT,</t> and TERT cell using primer set 1. N = 3. (G) This is similar to (F) but shows the results of primer set 2 in the same experiments. (H) This is similar to (F) but shows the expression of the senescence marker p16INK4A in the same experiments. (I) This is similar to (F) but shows the expression of the senescence marker p21WAF1 in the same experiments. (J) This is similar to (F) but shows SA-βGal expression. (K,L) Representative images of SA-βGal staining in (E). (K) Young growing NHOF-1 fibroblasts (20.2 MPDs). (L) . NHOF-1 cells (68.2 MPDs). Bar, 100 µm. (F–J) (A–E) Plain yellow bars, LATE PURO; blue horizontal striped bars, TERT-HA; brown stippled bars, DNTERT; and purple left-right diagonally striped bars, TERT all after completing 60 MPDs. *, P < 0.05; **, P < 0.01; ***, P < 0.001; * >0.05 < 0.1; ns, not significant. The results are presented as the averages +/- standard deviation. N = 4, except (J) .
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    ANKH transcript is upregulated in PEsen human fibroblasts and downregulated by telomerase. (A) Expression of ANKH mRNA in growing and PEsen NHOF-1 cells using primer set 1. (B) This is similar to (A) but shows the results of primer set 2 in the same experiments. (C) This is similar to (A) but shows the expression of the senescence marker p16INK4A in the same experiments. (D) This is similar to (A) but shows the expression of the senescence marker p21WAF1 in the same experiments. (E) This is similar to (A) but shows SA-βGal expression. (A–E) Yellow plain bars, growing young cells 20.2-21 MPDs; blue horizontal striped bars, PEsen cells 68.2 MPDs. *, P < 0.05; **, P < 0.01; ***, P < 0.001; *P > 0.05 < 0.1; ns, not significant. The results are presented as the averages +/- standard deviation. N = 3. (F) Expression of ANKH mRNA in NHOF-1 PURO, <t>TERT-HA,</t> <t>DNTERT,</t> and TERT cell using primer set 1. N = 3. (G) This is similar to (F) but shows the results of primer set 2 in the same experiments. (H) This is similar to (F) but shows the expression of the senescence marker p16INK4A in the same experiments. (I) This is similar to (F) but shows the expression of the senescence marker p21WAF1 in the same experiments. (J) This is similar to (F) but shows SA-βGal expression. (K,L) Representative images of SA-βGal staining in (E). (K) Young growing NHOF-1 fibroblasts (20.2 MPDs). (L) . NHOF-1 cells (68.2 MPDs). Bar, 100 µm. (F–J) (A–E) Plain yellow bars, LATE PURO; blue horizontal striped bars, TERT-HA; brown stippled bars, DNTERT; and purple left-right diagonally striped bars, TERT all after completing 60 MPDs. *, P < 0.05; **, P < 0.01; ***, P < 0.001; * >0.05 < 0.1; ns, not significant. The results are presented as the averages +/- standard deviation. N = 4, except (J) .
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    ANKH transcript is upregulated in PEsen human fibroblasts and downregulated by telomerase. (A) Expression of ANKH mRNA in growing and PEsen NHOF-1 cells using primer set 1. (B) This is similar to (A) but shows the results of primer set 2 in the same experiments. (C) This is similar to (A) but shows the expression of the senescence marker p16INK4A in the same experiments. (D) This is similar to (A) but shows the expression of the senescence marker p21WAF1 in the same experiments. (E) This is similar to (A) but shows SA-βGal expression. (A–E) Yellow plain bars, growing young cells 20.2-21 MPDs; blue horizontal striped bars, PEsen cells 68.2 MPDs. *, P < 0.05; **, P < 0.01; ***, P < 0.001; *P > 0.05 < 0.1; ns, not significant. The results are presented as the averages +/- standard deviation. N = 3. (F) Expression of ANKH mRNA in NHOF-1 PURO, <t>TERT-HA,</t> <t>DNTERT,</t> and TERT cell using primer set 1. N = 3. (G) This is similar to (F) but shows the results of primer set 2 in the same experiments. (H) This is similar to (F) but shows the expression of the senescence marker p16INK4A in the same experiments. (I) This is similar to (F) but shows the expression of the senescence marker p21WAF1 in the same experiments. (J) This is similar to (F) but shows SA-βGal expression. (K,L) Representative images of SA-βGal staining in (E). (K) Young growing NHOF-1 fibroblasts (20.2 MPDs). (L) . NHOF-1 cells (68.2 MPDs). Bar, 100 µm. (F–J) (A–E) Plain yellow bars, LATE PURO; blue horizontal striped bars, TERT-HA; brown stippled bars, DNTERT; and purple left-right diagonally striped bars, TERT all after completing 60 MPDs. *, P < 0.05; **, P < 0.01; ***, P < 0.001; * >0.05 < 0.1; ns, not significant. The results are presented as the averages +/- standard deviation. N = 4, except (J) .
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    retroviral vector backbone pmig  (Addgene inc)
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    ANKH transcript is upregulated in PEsen human fibroblasts and downregulated by telomerase. (A) Expression of ANKH mRNA in growing and PEsen NHOF-1 cells using primer set 1. (B) This is similar to (A) but shows the results of primer set 2 in the same experiments. (C) This is similar to (A) but shows the expression of the senescence marker p16INK4A in the same experiments. (D) This is similar to (A) but shows the expression of the senescence marker p21WAF1 in the same experiments. (E) This is similar to (A) but shows SA-βGal expression. (A–E) Yellow plain bars, growing young cells 20.2-21 MPDs; blue horizontal striped bars, PEsen cells 68.2 MPDs. *, P < 0.05; **, P < 0.01; ***, P < 0.001; *P > 0.05 < 0.1; ns, not significant. The results are presented as the averages +/- standard deviation. N = 3. (F) Expression of ANKH mRNA in NHOF-1 PURO, <t>TERT-HA,</t> <t>DNTERT,</t> and TERT cell using primer set 1. N = 3. (G) This is similar to (F) but shows the results of primer set 2 in the same experiments. (H) This is similar to (F) but shows the expression of the senescence marker p16INK4A in the same experiments. (I) This is similar to (F) but shows the expression of the senescence marker p21WAF1 in the same experiments. (J) This is similar to (F) but shows SA-βGal expression. (K,L) Representative images of SA-βGal staining in (E). (K) Young growing NHOF-1 fibroblasts (20.2 MPDs). (L) . NHOF-1 cells (68.2 MPDs). Bar, 100 µm. (F–J) (A–E) Plain yellow bars, LATE PURO; blue horizontal striped bars, TERT-HA; brown stippled bars, DNTERT; and purple left-right diagonally striped bars, TERT all after completing 60 MPDs. *, P < 0.05; **, P < 0.01; ***, P < 0.001; * >0.05 < 0.1; ns, not significant. The results are presented as the averages +/- standard deviation. N = 4, except (J) .
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    Image Search Results


    ANKH transcript is upregulated in PEsen human fibroblasts and downregulated by telomerase. (A) Expression of ANKH mRNA in growing and PEsen NHOF-1 cells using primer set 1. (B) This is similar to (A) but shows the results of primer set 2 in the same experiments. (C) This is similar to (A) but shows the expression of the senescence marker p16INK4A in the same experiments. (D) This is similar to (A) but shows the expression of the senescence marker p21WAF1 in the same experiments. (E) This is similar to (A) but shows SA-βGal expression. (A–E) Yellow plain bars, growing young cells 20.2-21 MPDs; blue horizontal striped bars, PEsen cells 68.2 MPDs. *, P < 0.05; **, P < 0.01; ***, P < 0.001; *P > 0.05 < 0.1; ns, not significant. The results are presented as the averages +/- standard deviation. N = 3. (F) Expression of ANKH mRNA in NHOF-1 PURO, TERT-HA, DNTERT, and TERT cell using primer set 1. N = 3. (G) This is similar to (F) but shows the results of primer set 2 in the same experiments. (H) This is similar to (F) but shows the expression of the senescence marker p16INK4A in the same experiments. (I) This is similar to (F) but shows the expression of the senescence marker p21WAF1 in the same experiments. (J) This is similar to (F) but shows SA-βGal expression. (K,L) Representative images of SA-βGal staining in (E). (K) Young growing NHOF-1 fibroblasts (20.2 MPDs). (L) . NHOF-1 cells (68.2 MPDs). Bar, 100 µm. (F–J) (A–E) Plain yellow bars, LATE PURO; blue horizontal striped bars, TERT-HA; brown stippled bars, DNTERT; and purple left-right diagonally striped bars, TERT all after completing 60 MPDs. *, P < 0.05; **, P < 0.01; ***, P < 0.001; * >0.05 < 0.1; ns, not significant. The results are presented as the averages +/- standard deviation. N = 4, except (J) .

    Journal: Frontiers in Aging

    Article Title: Membrane transporter progressive ankylosis protein homologue ( ANKH / Ank ) partially mediates senescence-derived extracellular citrate and is regulated by DNA damage, inflammation, and ageing

    doi: 10.3389/fragi.2025.1583288

    Figure Lengend Snippet: ANKH transcript is upregulated in PEsen human fibroblasts and downregulated by telomerase. (A) Expression of ANKH mRNA in growing and PEsen NHOF-1 cells using primer set 1. (B) This is similar to (A) but shows the results of primer set 2 in the same experiments. (C) This is similar to (A) but shows the expression of the senescence marker p16INK4A in the same experiments. (D) This is similar to (A) but shows the expression of the senescence marker p21WAF1 in the same experiments. (E) This is similar to (A) but shows SA-βGal expression. (A–E) Yellow plain bars, growing young cells 20.2-21 MPDs; blue horizontal striped bars, PEsen cells 68.2 MPDs. *, P < 0.05; **, P < 0.01; ***, P < 0.001; *P > 0.05 < 0.1; ns, not significant. The results are presented as the averages +/- standard deviation. N = 3. (F) Expression of ANKH mRNA in NHOF-1 PURO, TERT-HA, DNTERT, and TERT cell using primer set 1. N = 3. (G) This is similar to (F) but shows the results of primer set 2 in the same experiments. (H) This is similar to (F) but shows the expression of the senescence marker p16INK4A in the same experiments. (I) This is similar to (F) but shows the expression of the senescence marker p21WAF1 in the same experiments. (J) This is similar to (F) but shows SA-βGal expression. (K,L) Representative images of SA-βGal staining in (E). (K) Young growing NHOF-1 fibroblasts (20.2 MPDs). (L) . NHOF-1 cells (68.2 MPDs). Bar, 100 µm. (F–J) (A–E) Plain yellow bars, LATE PURO; blue horizontal striped bars, TERT-HA; brown stippled bars, DNTERT; and purple left-right diagonally striped bars, TERT all after completing 60 MPDs. *, P < 0.05; **, P < 0.01; ***, P < 0.001; * >0.05 < 0.1; ns, not significant. The results are presented as the averages +/- standard deviation. N = 4, except (J) .

    Article Snippet: pBABE retroviral vectors on the puromycin-resistant backbone expressing TERT and TERT-HA , the dominant-negative catalytically dead TERT DNTERT , or the empty vector were obtained from Addgene Europe (Teddington, Middlesex, UK; Cat# 1771, 1772 and 1775, respectively).

    Techniques: Expressing, Marker, Standard Deviation, Staining